simpliamp tm thermal cycler (Thermo Fisher)

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Thermo Fisher simpliamp tm thermal cycler
Simpliamp Tm Thermal Cycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/simpliamp+tm+thermal+cycler/simplamp+thermal+cycler/pmc12174097-130-10-14
Average 90 stars, based on 1 article reviews

simpliamp tm thermal cycler - by Bioz Stars, 2026-10

90/100 stars

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Polymerase Chain Reaction:

Article Title: Myxoid glioneuronal tumor: Histopathologic, neuroradiologic, and molecular features in a single center series.
Article Snippet: Background: Myxoid glioneuronal tumor (MGT) is a benign glioneuronal neoplasm recently introduced in the World Health Organization (WHO) classification of the central nervous system (CNS) tumors.. MGTs are typically located in the septum pellucidum, foramen of Monro or periventricular white matter of the lateral ventricle.. They were previously diagnosed as dysembryoplastic neuroepithelial tumors (DNT), showing histological features almost indistinguishable from classical cortical DNT.

Article Title: Transcriptomic Insights and the Development of Microsatellite Markers to Assess Genetic Diversity in the Broodstock Management of Litopenaeus stylirostris
Article Snippet: The first PCR amplification was carried out in a reaction volume of 10 μL comprising a 5 μL 2× Taq DNA polymerase Mix (Bioman, Taipei, Taiwan), 0.3 μL forward primer, 0.3 μL reverse primer, 2.4 μL 2dH 2 O, and 2 μL template DNA. .. PCR was performed using a 96-well polymerase chain reactor Veriti ® thermal cycler or SimpliAMP TM thermal cycler (both from Applied Biosystems, Foster City, CA, USA) and the following reaction conditions: 94 °C for 5 min; 30 cycles of 94 °C for 40 s, T m °C for 30 s, and 72 °C for 40 s; 72 °C for 8 min; and maintaining the temperature at 4 °C. ..

Purification:

Sequencing:

Amplification:

Article Title: NOS3 rs3918188C>A is associated with susceptibility to resistant hypertension while CES1 genetic variation was not associated with resistant hypertension among South Africans
Article Snippet: All PCRs were performed in a 25 μL reaction volume containing 100 ng genomic DNA; 1X Green GoTaq Flexi ® Reaction Buffer (Promega Corporation, Madison, WI, United States); 0.4 μM of deoxynucleotide triphosphates (dNTPs) (Promega Corporation, Madison, WI, United States); 1.5 mM magnesium chloride (Promega Corporation, Madison, WI, United States); 0.2 μM each of forward and reverse primers (Inqaba Biotechnical Industries (Pty) Ltd., South Africa), 1 U of GoTaq Flexi ® DNA Polymerase (Promega Corporation, Madison, WI, United States) and nuclease-free water. .. Amplification was done using a SimpliAmp TM Thermal Cycler (Applied BiosystemsTM, Thermofisher Scientific, Massachusetts, United States). .. The reaction conditions were an initial denaturation at 94°C for 3 min; followed by 35 cycles of further denaturation at 94°C for 30 s; annealing at temperatures specific for each SNP ( ) for 30 s; initial extension at 72°C for 30 s; and final extension at 72°C for 10 min. PCR products from amplification of regions flanking the NOS3 rs1799983G>T and CES1 rs2244613G>T SNPs, were digested using BanI and AlwN1 restriction enzymes, respectively (New England BioLabs ® , Ipswich, United Kingdom).

Reverse Transcription:

Article Title: The Effect of the Root Bark of Lycium chinense (Lycii Radicis Cortex) on Experimental Periodontitis and Alveolar Bone Loss in Sprague-Dawley Rats
Article Snippet: .. An amount of 2 μg of RNA was reverse transcribed using oligo (dT) primer, Accupower ® RT PreMix (Bioneer, Daejeon, Republic of Korea), and a SimpliAmp TM Thermal Cycler (Applied Biosystems, Waltham, MA, USA). .. Synthesized cDNA was amplified using SyBr green Ex-Taq master mix (Takara, Shiga, Japan), Quantstudio 5 Thermal cycler (Applied Biosystems), and specific primers for inducible nitric oxide synthase ( iNOS ), cyclooxygenase-2 ( COX-2 ), tumor necrosis factor-α ( TNF-α ), interleukin ( IL )- 1β , monocyte chemoattractant protein-1 ( MCP-1 ), and glyceraldehyde-3-phosphate dehydrogenase ( GAPDH ).

Article Title: In Vitro Assessment of Anti-Adipogenic and Anti-Inflammatory Properties of Black Cumin ( Nigella sativa L.) Seeds Extract on 3T3-L1 Adipocytes and Raw264.7 Macrophages
Article Snippet: Total RNA from the treated cells was isolated using Trizol solution (Invitrogen, Carlsbad, CA, USA) and quantified using a NanoDrop (ThermoFisher Scientific, Waltham, MA, USA). .. Subsequently, 2 μg of RNA was reverse-transcribed into cDNA using oligo-d(T)16 primers, Accupower ® RT PreMix (Bioneer, Daejeon, Republic of Korea), and a SimpliAmp TM thermal cycler (Applied Biosystems, Waltham, MA, USA). .. The qPCR was conducted with gene-specific primers targeting iNOS, COX-2, PPARγ, C/BEPα, aP2, LPL, FAS, and monocyte chemoattractant protein-1 (MCP-1), SREBP-1c, TNF-α, IL-6, IL-1β ( ) using the diluted cDNA (50 ng), SyBr Green Ex Taq (Takara, Shiga, Japan), and the QuantStudio 5 real-time PCR instrument (Applied Biosystems, Waltham, MA, USA).

Nested PCR:

Article Title: Molecular Surveillance for Potential Zoonotic Pathogens in Troglophilus Bats: Detection and Molecular Characterization of Bat Coronaviruses in Southern Italy
Article Snippet: .. Nested PCR assays were carried out in a SimpliAmp TM thermal cycler (Applied Biosystem, Thermo Fisher Scientific Inc., Waltham, MA, USA). .. Reverse transcription and first round amplification (pan-CoV first) were carried out in a one-step protocol using the QIAGEN ® OneStep RT-PCR Kit (Qiagen S.p.A, Hilden, Germany) in a 25 μL mix containing 5 μL of 5x QIAGEN OneStep RT-PCR Buffer, 2.5 μL of each primer (10 μM) Hu-F and Hu-R, 1 μL of (10 mM) dNTP Mix, 0.25 μL of RNase Inhibitor (40 U/μL; Euroclone S.p.A., Pero, Italy), 5 μL of RNA extract, and 7.75 μL of nuclease-free water.

Incubation:

Article Title: Genetic Variation in ABCB1 , ADRB1 , CYP3A4 , CYP3A5 , NEDD4L and NR3C2 Confers Differential Susceptibility to Resistant Hypertension among South Africans
Article Snippet: Each digest reaction was carried out in a 30 μL reaction volume containing, 10 μL of PCR product, 1X CutSmartTM Buffer (New England BioLabs ® , Ipswich, UK), 3U restriction enzyme specific for each SNP, and nuclease-free water. .. All restriction enzyme digest reactions were incubated and inactivated on a SimpliAmp TM Thermal Cycler (Applied Biosystems, ThermoFisher Scientific, Life Technologies, Singapore) at temperatures and periods specific for each enzyme according to the manufacturer’s protocol ( https://nebcloner.neb.com/#!/redigest (accessed on 16 May 2024)). .. Restriction enzyme digests were resolved using electrophoresis on a 3% ( w / v ) agarose gel stained with SafeView TM Classic (Applied Biological Materials Inc., Richmond, BC, Canada) nucleic acid stain.

Article Title: NOS3 rs3918188C>A is associated with susceptibility to resistant hypertension while CES1 genetic variation was not associated with resistant hypertension among South Africans
Article Snippet: In each 30 μL digest reaction, 10 μL of PCR product, 1X CutSmartTM Buffer (New England BioLabs ® , Ipswich, United Kingdom), 3 U of restriction enzyme specific for each SNP, and nuclease-free water were added. .. Restriction enzyme digest reactions were incubated and inactivated on a SimpliAmp TM Thermal Cycler (Applied BiosystemsTM, Thermofisher Scientific, Massachusetts, United States) at temperatures and periods specific for each enzyme according to the manufacture’s protocol [ https://nebcloner.neb.com/#!/redigest (last accessed on 10 February 2025)] and resolved using electrophoresis on a 3% (w/v) agarose gel. ..

Electrophoresis:

Article Title: NOS3 rs3918188C>A is associated with susceptibility to resistant hypertension while CES1 genetic variation was not associated with resistant hypertension among South Africans
Article Snippet: In each 30 μL digest reaction, 10 μL of PCR product, 1X CutSmartTM Buffer (New England BioLabs ® , Ipswich, United Kingdom), 3 U of restriction enzyme specific for each SNP, and nuclease-free water were added. .. Restriction enzyme digest reactions were incubated and inactivated on a SimpliAmp TM Thermal Cycler (Applied BiosystemsTM, Thermofisher Scientific, Massachusetts, United States) at temperatures and periods specific for each enzyme according to the manufacture’s protocol [ https://nebcloner.neb.com/#!/redigest (last accessed on 10 February 2025)] and resolved using electrophoresis on a 3% (w/v) agarose gel. ..

Agarose Gel Electrophoresis:

Article Title: NOS3 rs3918188C>A is associated with susceptibility to resistant hypertension while CES1 genetic variation was not associated with resistant hypertension among South Africans
Article Snippet: In each 30 μL digest reaction, 10 μL of PCR product, 1X CutSmartTM Buffer (New England BioLabs ® , Ipswich, United Kingdom), 3 U of restriction enzyme specific for each SNP, and nuclease-free water were added. .. Restriction enzyme digest reactions were incubated and inactivated on a SimpliAmp TM Thermal Cycler (Applied BiosystemsTM, Thermofisher Scientific, Massachusetts, United States) at temperatures and periods specific for each enzyme according to the manufacture’s protocol [ https://nebcloner.neb.com/#!/redigest (last accessed on 10 February 2025)] and resolved using electrophoresis on a 3% (w/v) agarose gel. ..

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